primary human umbilical cord mesenchymal stem cells huc mscs  (PromoCell)

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: METTL1-deficient MSCs display reduced proliferation and increased susceptibility to senescence and apoptosis. (A, B) Evaluation of METTL1 mRNA and protein expression in MSCs transduced with lentiviruses containing shRNA targeting METTL1. (C) CCK-8 assays comparing the proliferation of MSC shGFP and MSC shMETTL1 . (D) Representative images of EdU staining in MSC shGFP and MSC shMETTL1 cells were captured following a 24-hour incubation with FFA (scale bars = 100 μm). (E) Representative images of SA-β-gal staining were performed using a senescence β-galactosidase staining kit in the indicated groups (scale bars = 100 μm). (F, G) Cell cycle analysis of MSC shGFP and MSC shMETTL1 , presented for the G0/G1, S, and G2/M phases in the respective groups. (H, I) Analysis of cell apoptosis was conducted in MSC shGFP and MSC shMETTL1 under conditions of FFA exposure or without. All statistical analyses are presented in the right panel of the data. For all statistical plots, data are expressed as mean ± S.E.M, and statistical significance is indicated in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Expressing, Transduction, shRNA, CCK-8 Assay, Staining, Incubation, Cell Cycle Assay

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: METTL1-deficient MSCs inhibit lipid synthesis in hepatocytes. (A) Representative images of Nile Red staining in hepatocytes co-cultured with MSC shGFP and MSC shMETTL1 following treatment with FFA (Scale bar = 20 μm). (B) Measurement of TG content in hepatocytes in the indicated groups. (C, D) Western blot analysis of lipid metabolism-related gene expression (FASN, SREBP1, SCD1) in the indicated groups. (E, F) qPCR analysis of lipid synthesis gene expression ( Fasn, Scd1, Srebp1, Fads1 and Acaca ) in AML12 and HepG2 cells co-cultured with MSC shGFP and MSC shMETTL1 . For all statistical graphs, data are presented as mean ± S.E.M, with statistical significance is indicated in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Staining, Cell Culture, Western Blot, Gene Expression

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: Transplantation of METTL1-deficient MSCs alleviates metabolic disorders associated with MASLD. (A) Schematic diagram of the animal experiment. (B) Evaluation of liver weight and the liver-to-body weight ratio in the indicated mice. (C) Assessment of fasting blood glucose levels in the indicated mice. (D) Analysis of GTT and ITT for the indicated groups. (E) Measurement of serum ALT and AST levels following 7 weeks of cell transplantation. (F) Representative images of HE and Oil Red O staining for analysis of mouse liver tissue (Scale bar = 100 μm). (G) Determination of TG and TC levels in the liver tissue of the specified mice. (H) qPCR analysis of lipid synthesis-related genes, including Fasn, Scd1, Srebp1, Fads1 , and Acaca in the specified groups. (I) Western blot analysis of lipid metabolism-related proteins in the specified groups. For all statistical graphs, individual data points represent individual mice, and data are presented as mean ± S.E.M. Statistical significance is indicated as shown in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Transplantation Assay, Staining, Western Blot

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: METTL1-deficient MSCs exhibit elevated NAMPT secretion. (A) Volcano plot depicting differentially expressed genes between MSC shGFP and MSC shMETTL1 , as analyzed by RNA sequencing. (B) Identification of genes significantly enriched in GO analysis for MSC shMETTL1 cells. (C) Venn diagram illustrating the distinct proteins present in the secretome of MSC shGFP and MSC shMETTL1 . (D) GO analysis of proteins that are significantly enriched in the secretome of MSC shMETTL1 cells. (E) Heatmap representation of differentially expressed secretory proteins implicated in the regulation of lipid metabolism associated with MASLD in MSC shGFP and MSC shMETTL1 . (F) qPCR analysis of the expression of relevant genes in the specified cells. (G) ELISA measurements of the levels of differentially expressed secretory proteins in the supernatants of MSC shGFP and MSC shMETTL1 cells. For all statistical graphs, individual data points represent independent experimental replicates, and data are presented as mean ± S.E.M. Statistical significance is indicated as shown in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: RNA Sequencing, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: NAMPT/SIRT1/SREBP1 mediates the protective effects of METTL1-deficient MSCs in MASLD. (A-C) Representative images of IF staining for NAMPT, SIRT1 and SREBP1 in HepG2 cells co-cultured with MSC shGFP and MSC shMETTL1 cells after treatment with FFA (Scale bar = 20 μm). (D, E) Western blot analysis of NAMPT, SIRT1 and SREBP1 expression in HepG2 cells co-cultured with MSC shGFP and MSC shMETTL1 cells following FFA treatment. (F) The NAD+ content was measured in the indicated cells. (G-I) Representative images of IF staining for NAMPT, SIRT1 and SREBP1 in liver tissues from mice transplanted with MSC shGFP and MSC shMETTL1 cells after 16 weeks of HFD feeding (Scale bar = 100 μm). (J, K) Western blot analysis of NAMPT, SIRT1, and SREBP1 expression in liver tissues from mice transplanted with MSC shGFP and MSC shMETTL1 cells after 16 weeks of HFD feeding. (L) The NAD+ content was measured in the indicated mouse liver tissues. For all statistical graphs, data are presented as mean ± S.E.M., with statistical significance indicated in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Staining, Cell Culture, Western Blot, Expressing

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: FK866 primed METTL1-deficient MSCs fail to protect against MASLD-related metabolic disorders due to impaired NAMPT secretion. (A) Schematic diagram of the animal experiment. (B) Assessment of liver weight and the liver-to-body weight ratio in the indicated mice. (C) Evaluation of fasting blood glucose levels in the specified mice. (D) GTT and ITT analyses were conducted on the designated groups. (E) Measurement of serum ALT and AST levels was conducted 7 weeks post-cell transplantation. (F) Representative images of HE and Oil Red O staining of mouse liver tissues (Scale bar = 100 μm). (G) Determination of TG and TC levels in the liver tissues. (H) Western blot analysis was performed to investigate the proteins associated with lipid metabolism and NAMPT/SIRT1 signaling in mouse liver tissues. For all statistical graphs, individual data points represent individual mice, and data are presented as mean ±S.E.M. Statistical significance is indicated as shown in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Transplantation Assay, Staining, Western Blot

Journal: Stem Cells Translational Medicine

Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

doi: 10.1093/stcltm/szag016

Figure Lengend Snippet: METTL1-overexpressing MSCs accelerate MASLD progression with reduced NAMPT secretion. (A) Schematic diagram of the animal experiment. (B) GTT and ITT analyses were conducted on the designated groups. (C) Representative images of HE and Oil Red O staining of mouse liver tissues (Scale bar = 100 μm). (D) Measurement of TG and TC levels in the liver tissues. (E) qPCR analysis of lipid synthesis-related genes in the designated groups. (F) Western blot analysis was performed to investigate the proteins associated with lipid metabolism and NAMPT/SIRT1 signaling in mouse liver tissues. For all statistical analyses, individual data points represent individual mice, and data are presented as mean ±S.E.M. Statistical significance is indicated as shown in the figure.

Article Snippet: Human umbilical cord-derived MSCs (CP-CL11) were obtained from Procell (China) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Staining, Western Blot

Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model

doi: 10.3389/fcell.2026.1784998

Figure Lengend Snippet: Characterization of ADSC EVs and UC-MSC EVs. (a) Size and concentration measurement by NTA; (b) Morphology visualization with Cryo-TEM with EVs indicated by red arrows (scale bar: 100 nm); (c) EV related biomarkers in the EV groups and cell lysate (CL) detected with Western blot, including positive markers TSG101, HSP70, CD63 and negative marker calnexin.

Article Snippet: The EVs used in this study were produced by EchoBiotech (Beijing, China), using commercial human cell lines UC-MSC (ATCC #PCS-500-010) and ADSC (ATCC #PCS-500-011).

Techniques: Concentration Assay, Western Blot, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model

doi: 10.3389/fcell.2026.1784998

Figure Lengend Snippet: Bulk transcriptomic analysis of a reconstructed skin model after treatment with ADSC EVs and UC-MSC EVs. (a,b) Volcano plots and heatmaps showing commonly DEGs regulated by high and low doses of (a) ADSC EVs and (b) UC-MSC EVs, with 5-8 replicates per group; (c,d) Top GO terms enriched among DEGs modulated by (c) ADSC EVs and (d) UC-MSC EVs, as identified in the GO database.

Article Snippet: The EVs used in this study were produced by EchoBiotech (Beijing, China), using commercial human cell lines UC-MSC (ATCC #PCS-500-010) and ADSC (ATCC #PCS-500-011).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model

doi: 10.3389/fcell.2026.1784998

Figure Lengend Snippet: Comprehensive profiling of miRNA and protein cargo in EVs. (a) Venn diagram illustrating miRNAs in the two groups of EVs; (b) Top abundantly expressed miRNAs identified in each EV types; (c) Overlap between predicted target genes of shared miRNAs and DEGs from T-skin™ transcriptomic analysis; (d) Identified proteins in the two groups of EVs revealed by Venn plot; (e) Top expressed proteins in the two types of EVs; (f) Selected DEPs that upregulated in ADSC EVs compared to UC-MSC EVs.

Article Snippet: The EVs used in this study were produced by EchoBiotech (Beijing, China), using commercial human cell lines UC-MSC (ATCC #PCS-500-010) and ADSC (ATCC #PCS-500-011).

Techniques: